Polyploidy in tissues of plants in vitro of grape somaclones

In vitro experimental plants obtained by clonal micropropagation of 9 grape somaclones 5 original forms were the material for cytogenetic research. A biological microscope XSP-146TP was used analysis. 823 cases deviation from diploidy observed in total. Significant tissue ploidy meristematic colchicine treatment proembryogenic cells various varieties. The significant direct correlation found between frequency polyploidy meristem tissues and number chloroplasts stomata somaclones. reverse on leaf area. Somaclone No. 72, as a result regeneration colchicinated Ruta variety identified tetraploid (2n = 4x 76), is recommended use polyploid creation program.